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Innovagen AB
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Innovagen AB
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Image Search Results
Journal: Antimicrobial Agents and Chemotherapy
Article Title: Treatment with Entinostat Heals Experimental Cholera by Affecting Physical and Chemical Barrier Functions of Intestinal Epithelia
doi: 10.1128/AAC.02570-16
Figure Lengend Snippet: CAP-18 peptide/protein expression in the ileal mucosae of healthy rabbits, Vibrio cholerae-infected rabbits, and rabbits that were infected and treated with entinostat. Mucosal sections of ileum from healthy rabbits (n = 5), V. cholerae-infected rabbits (n = 5), and infected rabbits treated with 0.5 mg entinostat twice a day for 2 days (n = 5) or with 1 mg entinostat once a day for 2 days (n = 5) were stained with a CAP-18-specific antibody. (A) Representative photomicrographs. CAP-18-positive staining is brown; cell nuclei are stained blue with hematoxylin. (Top left) Prominent expression of CAP-18 (thick arrows) in the SE of healthy ileal mucosae. (Top right) Downregulation of CAP-18 expression in the SE after V. cholerae infection. Partial erosion of SE (arrowheads) was also noted. (Bottom) Recovery of epithelial erosion and restoration of CAP-18 expression (arrows) in the SE after treatment with 0.5 mg entinostat twice a day for 2 days (left) or 1 mg entinostat once a day for 2 days (right). Bars, 50 μm (top and bottom left); 100 μm (bottom right). (B) Semiquantitative expression of CAP-18 in SE, given as ACIA scores (see Materials and Methods). Data are means ± SEM. Differences in outcomes between different groups were estimated by mixed-model ANOVA. Differences are significant at a P value of <0.05.
Article Snippet: The affinity-purified
Techniques: Expressing, Infection, Staining
Journal: Antimicrobial Agents and Chemotherapy
Article Title: Treatment with Entinostat Heals Experimental Cholera by Affecting Physical and Chemical Barrier Functions of Intestinal Epithelia
doi: 10.1128/AAC.02570-16
Figure Lengend Snippet: In vitro inhibitory effect of entinostat or CAP-18 on the growth of Vibrio cholerae. A suspension of V. cholerae Ogawa was incubated with different concentrations of entinostat (A) or synthetic CAP-18 (B) in MHB in wells of a microtiter plate at 37°C for 2 h. Bacterial suspensions from individual wells were plated on TTGA, and CFU were counted after overnight incubation. Data are means ± SEM from 2 and 4 individual experiments for entinostat and CAP-18, respectively.
Article Snippet: The affinity-purified
Techniques: In Vitro, Incubation